Figure 1. HIF1A-AS2 was upregulated in osteosarcoma and was related to poor survival. (A) The expression of HIF1A-AS2 in 30 osteosarcoma samples and their noncancerous pairs was detected by qRT-PCR. U6 was used as the internal control. (B) The expression of HIF1A-AS2 was upregulated in 23 cases (23/30; 77 %) compared to the expression in adjacent tissues. (Defined as a cutoff of Log 2.0-fold-change >1) (C) The high HIF1A-AS2 expression group had a shorter overall survival than the low HIF1A-AS2 expression group (median overall survival =50 vs. 92 months, respectively; log-rank p<0.01).