Figure 3. Ectopic expression of HIF1A-AS2 inhibited miR-129-5p expression. (A) The expression of miR-129-5p in MG-63 cells was detected by using qRT-PCR analysis. (B) The binding sites between HIF1A-AS2 and miR-129-5p obtained from bioinformatics analysis are shown. (C) We showed that overexpression of miR-129-5p can decrease the luciferase activity of wild-type (WT) HIF1A-AS2 but not mutant HIF1A-AS2. (D) Overexpression of miR-129-5p decreased the expression of HIF1A-AS2 in MG-63 cells. (E) Ectopic expression of HIF1A-AS2 suppressed miR-129-5p expression in MG-63 cells. (F) Overexpression of HIF1A-AS2 increased VCP expression in MG-63 cells. (G) The protein expression of VCP was detected by Western blot. GAPDH was used as the control. *p<0.05.