Figure 4. miR-129-5p was downregulated in osteosarcoma and was negatively related to HIF1A-AS2 expression. (A) The miR-129-5p expression in 30 osteosarcoma samples and their noncancerous pairs was determined by qRT-PCR. U6 was used as the internal control. (B) The expression of miR-129-5p was downregulated in 22 cancerous tissues (22/30; 73%) compared to the adjacent tissues. (Defined as a cutoff of Log 2.0-fold-change >1) (C) miR-129-5p expression was negatively correlated with HIF1A-AS2 expression in osteosarcoma samples by using Pearson's correlation coefficient analysis.