Figure 4. Downregulation of FADS1 induces apoptosis in PIG1 melanocytes. PIG1 cells were transfected with NC-siRNA or FADS1-siRNA2 for 72 h. (A) Cell viability determination (CCK-8 assay). (B, C) Ki67 immunofluorescence. (D, E) Cell death (trypan blue) assay. (F, G) Apoptosis assay. Annexin V/PI-stained cells were analyzed by flow cytometry. (H, I) Expression of FADS1 by western blot. **P < 0.01, compared with NC cells.