Figure 5. Downregulation of FADS1 decreased melanogenesis in PIG1 melanocytes. PIG1 cells were transfected with NC-siRNA or FADS1-siRNA2 for 72 h. (A) Melanin content determination (ELISA). (B) Expression of MITF, Tyrosinase, TRP1, and TRP2 detected by western blotting. β-actin was used as internal control. (C–F) Relative expression of MITF, Tyrosinase, TRP1, and TRP2 after normalization to β-actin. **P < 0.01, compared with NC cells.