Research Paper Volume 11, Issue 24 pp 11844—11864

Hydrogen sulfide attenuates mitochondrial dysfunction-induced cellular senescence and apoptosis in alveolar epithelial cells by upregulating sirtuin 1

Figure 4. Effects of NaHS on mitochondrial function in CSE-stimulated A549 cells. A549 cells were cultured with and without 3% CSE and/or 100, 200, or 400μM NaHS for 48 h. (A) The bioenergetic profiles of A549 cells were measured by a Seahorse Extracellular Flux Analyzer, OCR in cells treated with oligomycin, FCCP, and rotenone and Antimycin A. (B) Quantitative analysis of basal respiration, ATP production, maximal respiratory and spare capacity is shown. (C) mtDNA copy number was measured by Real-time PCR. (D) The mRNA levels of COXI, COXII, COXIII, ATPase 6 and Cyto b were detected using Real-time PCR. *P<0.05, **P<0.01, significantly different from control cells [3%CSE (-) and NaHS (-)]; #P<0.05, ##P<0.01, significantly different from cells treated with 3%CSE only.