Research Paper Volume 11, Issue 24 pp 11844—11864

Hydrogen sulfide attenuates mitochondrial dysfunction-induced cellular senescence and apoptosis in alveolar epithelial cells by upregulating sirtuin 1

Figure 7. Effects of SIRT1 on the NaHS-mediated mitochondrial damage in CSE-stimulated A549 cells. A549 cells were cultured with SIRT1 inhibitor (EX527) in the absence and presence of 3% CSE and NaHS for 48 h. (A) The bioenergetic profiles of A549 cells were measured by a Seahorse Extracellular Flux Analyzer, OCR in cells treated with oligomycin, FCCP, and rotenone and Antimycin A. (B) Quantitative analysis of basal respiration, ATP production, maximal respiratory and spare capacity is shown. (C) Mitochondrial permeability potential was determined by JC-1 staining. Red fluorescence represented normal membrane potential, and green fluorescence represented mitochondrial membrane potential depolarization. *P<0.05, **P<0.01, significantly different from control cells [CSE (-), NaHS (-) and EX 527 (-)]; #P<0.05, ##P<0.01, significantly different from cells treated with 3% CSE only; &&P<0.01, significantly different from cells treated with EX 527 only.