Research Paper Volume 11, Issue 24 pp 11905—11921

KIF20A promotes cellular malignant behavior and enhances resistance to chemotherapy in colorectal cancer through regulation of the JAK/STAT3 signaling pathway

Figure 6. KIF20A induces chemo-resistance in CRC. (A, C) Western blot analysis of apoptosis-related factors (cleaved-caspase 3, bax, bcl-2) in different transfected groups in HCT-116 cell line treated with 4ug/ml 5-FU (left) or 8 ug/ml oxaliplatin (right) and LOVO cell line treated with 1.5 ug/ml 5-FU (left) or 1 ug/ml oxaliplatin (right). (B, D) Cell viability was measured using CCK-8 analysis and compared between different groups with different treatments at indicated times. (EH) The apoptotic rates of different transfected groups with different treatments were measured by flow cytometry. Data are presented as mean ± SEM. *P < 0.05; **P < 0.01.