Figure 6. KIF20A induces chemo-resistance in CRC. (A, C) Western blot analysis of apoptosis-related factors (cleaved-caspase 3, bax, bcl-2) in different transfected groups in HCT-116 cell line treated with 4ug/ml 5-FU (left) or 8 ug/ml oxaliplatin (right) and LOVO cell line treated with 1.5 ug/ml 5-FU (left) or 1 ug/ml oxaliplatin (right). (B, D) Cell viability was measured using CCK-8 analysis and compared between different groups with different treatments at indicated times. (E–H) The apoptotic rates of different transfected groups with different treatments were measured by flow cytometry. Data are presented as mean ± SEM. *P < 0.05; **P < 0.01.