Figure 2. Effects of Leu (0.5 mM or 10 mM), KIC (0.5 mM or 10 mM), and HMB (50 μM) on the gene expression of myosin heavy chain isoform (MyHC I, IIa, IIx, and IIb) (A) and the protein expression of MyHCI and MyHC IIb (B). Results are expressed as mean ± SEM. Different letters (a, b, c) indicated significant differences (P < 0.05). CON, control; HMB, β-hydroxy-β-methyl butyrate; KIC, α-ketoisocaproate; Leu, leucine.