Research Paper Volume 11, Issue 24 pp 11922—11936

Suppression of protein degradation by leucine requires its conversion to β-hydroxy-β-methyl butyrate in C2C12 myotubes

Figure 2. Effects of Leu (0.5 mM or 10 mM), KIC (0.5 mM or 10 mM), and HMB (50 μM) on the gene expression of myosin heavy chain isoform (MyHC I, IIa, IIx, and IIb) (A) and the protein expression of MyHCI and MyHC IIb (B). Results are expressed as mean ± SEM. Different letters (a, b, c) indicated significant differences (P < 0.05). CON, control; HMB, β-hydroxy-β-methyl butyrate; KIC, α-ketoisocaproate; Leu, leucine.