Figure 4. Hsa_circ_0006948 could serve as a miR-490-3p sponge in ESCC cells. (A) Top five miRNA targets are displayed. (B) A schematic drawing showing the putative binding sites of the miR-490-3p associated with hsa_circ_0006948. (C) Luciferase reporter assay for the luciferase activity of Luc-circ_0006948 WT or Luc-circ_0006948 mutant in cells cotransfected with miRNA mimics. Data are the mean±SD of three experiments. (D) Hsa_ciic_0006948 expression was negatively correlated with miR-490-3p expression in 153 patients with ESCC. (E and F) RNA immunoprecipitation (RIP) assays were performed using an anti-AGO2 antibody with the transfection of miR-490-3p mimics (miR-490-3p) or miR-NC in TE-1 and KYSE30 cells to detect hsa_circ_0006948 expression according to qRT-PCR. ***P<0.001.