Research Paper Volume 11, Issue 24 pp 11988—12001

CircRNA AFF4 promotes osteoblast cells proliferation and inhibits apoptosis via the Mir-7223-5p/PIK3R1 axis

Figure 5. CircRNA AFF4 functioned in MC3T3-E1 cells. (A) circRNA AFF4 levels measured by qRT-PCR. (B, C) Effects of circRNA AFF4 on cell proliferation assessed by CCK8 and Edu staining. (D) Cyclin D1 and Cyclin D3 expression assessed by Western blot. (E) Bcl-2 and Bax expression measured by Western blot. (F) Flow cytometry was used to assess MC3T3-E1 apoptosis after transfection with circRNA AFF4. (G, H) PIK3R1 levels after transfection with circRNA AFF4 measured by western blot and qRT-PCR.