Figure 3. Essential roles of circ-NF1-419 on proliferation, apoptosis, cell cycle and autophagy test. Influence on astrocyte proliferation (A), apoptosis (B), and cell cycle (C); Western blot analysis (D) showed the levels of Atg12, LC3A, LC3B in over-expressing circNF1-419-transfected rat astrocytes; (E) Transmission electron microscopic imaging showing autolysosomes (arrows) and autophagosomes with double-membraned autophagic vacuoles (arrowheads) in over-expression circNF1-419-transfected rat astrocyte. Scale bar: 5μm and 2μm. Data are presented as the means±SD of 3 independent experiments. *p <0.05 and **p <0.01 vs. the model group by one-way ANOVA, followed by the Holm-Sidak test.