Research Paper Volume 11, Issue 24 pp 12002—12031

Circular RNA NF1-419 enhances autophagy to ameliorate senile dementia by binding Dynamin-1 and Adaptor protein 2 B1 in AD-like mice

Figure 7. CircNF1-419 enhances autophagy by binding dynamin-1 and AP2B1 protein in vivo.circNF1-419 can pull-down dynamin-1 and AP2B1 protein (A, B), the proteins of dynamin-1 and AP2B1 quantified using Wes™ and the Simple Western from ProteinSimple; and RNA binding protein immunoprecipitation assay showed that the dynamin-1 and AP2B1 protein can pull down the circNF1-419 (C, D); the computer-aided molecular simulation demonstrated that the dynamin-1 and AP2B1 protein binds the circNF1-419 (E); the expression of dynamin-1 and AP2B1 protein in the whole brain tissues (F); CircNF1-419 improves the brain transcriptome in AD mice (G); CircNF1-419 levels in AAV viral transduction system with RNA interference and separately an over-expressing circNF1-419 (H). Data are presented as the means±SD of 3 independent experiments. *p <0.05 and **p < 0.01 vs. the model group by one-way ANOVA, followed by the Holm-Sidak test.