Research Paper Volume 11, Issue 24 pp 12032—12042

Compound 13 activates AMPK-Nrf2 signaling to protect neuronal cells from oxygen glucose deprivation-reoxygenation

Figure 2. Compound 13 inhibited OGD-R-induced apoptosis activation in SH-SY5Y neuronal cells and primary neurons. SH-SY5Y human neuronal cells (AD) or the primary murine hippocampal neurons (E and F) were pretreated (for 2h) with applied concentration (5/10 μM) of compound 13 (“C13”), followed by OGD-R stimulation for applied time periods, cell apoptosis was tested by the assays mentioned in the text. Bars stands for mean ± standard deviation (SD, n=5). * p<0.05 vs. “Mock” cells. #p<0.05 vs. cells with “OGD-R” treatment (no C13 pretreatment). Each experiment was repeated four times with similar results obtained.