Figure 4. The role of macrophage infiltration in septic AKI. (A) F4/80 immunofluorescent staining (×400); (B) gray value for F4/80 immunofluorescent staining; (C) protein expression of heparin binding protein, and gray value for the Western blot band; (D) Renal tissue mRNA expression of Il-6 and Tnf-α. n=6 per group. Data are shown as the mean ± SEM. Significance was calculated by one-way ANOVA with Tukey’s post hoc test, *: P<0.05. Abbreviations: CLP + vehicle, the group in which mice underwent the cecal ligation and puncture operation; CLP + heparin, the group in which mice were treated with heparin before the cecal ligation and puncture operation.