Research Paper Volume 11, Issue 24 pp 12177—12201

Effects of XIAP on high fat diet-induced hepatic steatosis: a mechanism involving NLRP3 inflammasome and oxidative stress

Figure 4. Impairment of XIAP enhances HFD-hepatic inflammation by up regulation of NLRP3 activity. (A) Representative images of immunohistochemical analysis for Caspase-8 in liver tissue sections. (B) Representative western blot analysis (left) and quantification (right) of the expression of Caspase-8 in liver tissues. (C) Representative western blot analysis (left) and quantification (right) of the expression of NLRP3 inflammasome (NLRP3, ASC, and Caspase-1) in liver tissues, and bands for pro-IL-18, mature-IL-18, pro-IL-1β and mature-IL-1β. For all bar plots shown, data are expressed as the mean ± SEM. n = 8 per group. ap < 0.05 vs. ItsiRNA/SCD or ItsiXIAP/SCD. bp < 0.05 vs. ItsiRNA/HFD.