Figure 5. Knockdown of XIAP promotes HFD-induced oxidative stress in liver mice. (A) Calculation of ROS intensity, MDA, H2O2, O2-, XO, iNOS levels, and SOD, TAC, CAT, GST and GSH-Px activity in hepatic tissue samples from each group of mice. (B and C) Representative western blot analysis of the expression of SOD1, HO-1, Nrf2, and quantification of SOD1, HO-1, Nrf2, NQO-1 and GCLM in liver samples. (D) Representative images of immunohistochemical analysis for Nrf2, HO-1 and SOD1 expression in liver tissue sections. For all bar plots shown, data are expressed as the mean ± SEM. n = 8 per group. ap < 0.05 vs. ItsiRNA/SCD or ItsiXIAP/SCD. bp < 0.05 vs. ItsiRNA/HFD.