Research Paper Volume 11, Issue 24 pp 12177—12201

Effects of XIAP on high fat diet-induced hepatic steatosis: a mechanism involving NLRP3 inflammasome and oxidative stress

Figure 6. XIAP silence enhances dyslipidemia in PA-treated cells in vitro. (A) AML-12 and primary hepatocytes were transfected with XIAP specific siRNA sequence for 24 h, followed by transfection efficacy calculation using qPCR and western blot analysis. AML-12 and primary hepatocytes were subjected to XIAP siRNA transfection for 24 h, and then cells were incubated with 250 μM of PA for additional 24 h, followed by further investigation. (B) Representative western blot and qPCR analysis of the expression of ACCα, FAS and SCD1 in PA-induced hepatocytes and AML-12 cell line. (C) Determination of cellular TG and TC levels in AML-12 and hepatocytes was next detected. (D) qPCR analysis for genes associated with fatty acid metabolism, including CD36, FATP1, FABP1, CPT-1α and PPARα, in cells treated as indicated. For all bar plots shown, data are expressed as the mean ± SEM. n = 8 per group. ap < 0.05 vs. siXIAP or siRNA/PA.