Research Paper Volume 11, Issue 24 pp 12177—12201

Effects of XIAP on high fat diet-induced hepatic steatosis: a mechanism involving NLRP3 inflammasome and oxidative stress

Figure 7. Inhibition of XIAP positively regulates PA-triggered inflammation in vitro. AML-12 and primary hepatocytes were transfected with XIAP siRNA for 24 h, followed by PA (250 μM) treatment for another 24 h. Then, further studies as exhibited were performed. (A and B) Representative western blot and qPCR detection showed the changes in mRNA and protein levels of NLRP3 inflammasome (NLRP3, ASC and Caspase-1) in PA-induced cells. For all bar plots shown, data are expressed as the mean ± SEM. n = 8 per group. ap < 0.05 vs. siRNA/PA.