Research Paper Volume 11, Issue 24 pp 12177—12201

Effects of XIAP on high fat diet-induced hepatic steatosis: a mechanism involving NLRP3 inflammasome and oxidative stress

Figure 8. Functional loss of XIAP contributes to PA-induced oxidative stress in vitro. AML-12 and primary hepatocytes were transfected with XIAP siRNA for 24 h, followed by PA (250 μM) treatment for additional 24 h. Subsequently, further studies were carried out. (A and B) Determination of cellular ROS production and cell viability. (C) Calculation of cellular MDA, H2O2, O2- levels, TAC, SOD, CAT, GST and GSH activities. (D) Western blot and qPCR analysis showed the alteration of SOD1, NQO-1, HO-1 and Nrf-2 in cells treated as described. For all bar plots shown, data are expressed as the mean ± SEM. n = 8 per group. ap < 0.05 vs. siRNA/PA.