Figure 1. CARD6 expression is up-regulated in the spinal dorsal horn of mice. Representative (A) RT-qPCR and (B) western blot analysis of CARD6 expression in lumbar spinal tissues. *p < 0.05 and **p < 0.01 vs the Sham group. (C) The mRNA and (D) protein expression levels of CARD6 in primary cultured astrocytes, oligodendrocytes, microglia cells and mouse microglia BV2 cells incubated with TNF-α (10 ng/ml) plus IFN-γ (10 ng/ml) or LPS (100 ng/ml) for 24 h by RT-qPCR and western blot analysis, respectively. *p < 0.05 and **p < 0.01 vs the Con group in the absence of any treatments. (E) RT-qPCR and (F) western blot analysis of CARD6 expression in spinal cord tissues. Data represented means ± SEM (n=6 each group).