Research Paper Volume 11, Issue 24 pp 12213—12235

Targeting CARD6 attenuates spinal cord injury (SCI) in mice through inhibiting apoptosis, inflammation and oxidative stress associated ROS production

Figure 1. CARD6 expression is up-regulated in the spinal dorsal horn of mice. Representative (A) RT-qPCR and (B) western blot analysis of CARD6 expression in lumbar spinal tissues. *p < 0.05 and **p < 0.01 vs the Sham group. (C) The mRNA and (D) protein expression levels of CARD6 in primary cultured astrocytes, oligodendrocytes, microglia cells and mouse microglia BV2 cells incubated with TNF-α (10 ng/ml) plus IFN-γ (10 ng/ml) or LPS (100 ng/ml) for 24 h by RT-qPCR and western blot analysis, respectively. *p < 0.05 and **p < 0.01 vs the Con group in the absence of any treatments. (E) RT-qPCR and (F) western blot analysis of CARD6 expression in spinal cord tissues. Data represented means ± SEM (n=6 each group).