Figure 2. CARD6 knockout accelerates SCI in mice. (A) Western blot analysis CARD6 protein expression in brain and lumbar spinal tissues from CARD6+/+ or CARD6-/- mice. (B) The BMS scores and (C) BMS subscores were measured in each group of mice. (D) The withdrawal threshold was measured to calculate the mechanical hypersensitivity in each group of mice. (E) The withdrawal latency was measured to determine the thermal hypersensitivity in each group of mice. (F) H&E staining of adjacent sections. Scale bar: 100 μm. (G) Nissl staining of lumbar spinal cords in the ventral horn of gray matter from mice 3 days after SCI. The number of survived neuron was quantified. Scale bar: 100 μm (black arrows: the normal surviving neurons). (H) IF staining of GFAP and Iba-1 in the spinal dorsal horn of mice. Scale bar: 100 μm. (I) Quantification of GFAP and Iba-1 following IF analysis. (J) Western blot analysis of GFAP and Iba-1 in the lumbar spinal cord segments. (K, L) 5-HT and NeuN staining of axons in white matter tracts at the site of cord compression. (M) BDA-labeled the corticospinal tracts in proximity of the lesion sites of CARD6+/+ or CARD6-/- mice after SCI for 3 days. Data represented means ± SEM (n=8 each group). *p < 0.05 and **p < 0.01.