Research Paper Volume 11, Issue 24 pp 12213—12235

Targeting CARD6 attenuates spinal cord injury (SCI) in mice through inhibiting apoptosis, inflammation and oxidative stress associated ROS production

Figure 7. CARD6 silence-accelerated apoptosis and inflammation are regulated by ROS production in BV2 cells exposed to LPS. (AD) BV2 cells in the presence or absence of siCARD6 transfection were pre-treated with NAC (5 mM) or PLM (10 μM) for 2 h, and then were subjected to LPS (100 ng/ml) exposure for another 24 h. Subsequently, all cells were harvested for further analysis. (A) TUNEL and Cyto-c staining of BV2 cells. TUNEL- and Cyto-c-positive cells were quantified. Scale bar: 100 μm. (B) Protein expression levels of Cyto-c, Bcl-2, Bax and cleaved Caspase-3 were evaluated by western blot analysis. (C) RT-qPCR analysis of TNF-α and IL-1β in cells. (D) Protein expression levels of phosphorylated NF-κB in whole cells and NF-κB in nuclear were measured by western blot analysis. Data represented means ± SEM (n=6 each group). *p < 0.05 and **p < 0.01.