Research Paper Volume 11, Issue 24 pp 12328—12344

Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis

Figure 1. The expression levels of METTL1 and miR-149-3p in CR-CC cell lines. (A) The schematic procedures of the induction of CR-HCT116, CR-SW480 and CR-SW620 respectively. CCK-8 assay was conducted to detect (B) HCT116 cells, (C) SW480 cells and (D) SW620 cells proliferation respectively. Real-Time qPCR was performed to determine the levels of (E) miR-149-3p and (F) METTL1 mRNA in CC cells. (G, H) Western Blot was used to determine the expression levels of METTL1 in Cisplatin-sensitive colon cancer (CS-CC) and Cisplatin-resistant colon cancer (CR-CC) cells. (I) Real-Time qPCR was employed to investigate the effects of METTL1 on miR-149-3p levels in CR-CC cells. All the experiments repeated at least 3 times. “*” means p < 0.05, “**” means p < 0.01 and “NS” means no statistical significance.