Research Paper Volume 11, Issue 24 pp 12328—12344

Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis

Figure 2. METTL1 regulated CR-CC cell proliferation by targeting miR-149-3p. (A, B) Colony formation assay was performed to evaluate the colony formation abilities in CC cells. Western Blot was conducted to detect the expression levels of Cyclin D1, CDK2 and p27 in (C, D) CR-HCT116 cells, (E, F) CR-SW480 cells and (G, H) CR-SW620 cells. (“OE-METTL1” represented “Overexpressed METTL1 group”, and “OE-METTL1+Inhi” represented “Overexpressed METTL1 plus miR-149-3p inhibitor group”). All the experiments repeated at least 3 times. “*” means p < 0.05, “**” means p < 0.01 and “NS” means no statistical significance.