Research Paper Volume 11, Issue 24 pp 12328—12344

Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis

Figure 5. The effects of S100A4 and p53 on CR-CC cell viability. (A, B) TUNEL assay was conducted to detect cell apoptosis ratio in CC cells. (C, D) Western Blot was used to determine the expression levels of cleaved Caspase-3 in CR-CC cells. CCK-8 assay was performed to detect the proliferation ability of (E) CR-HCT116 cells, (F) CR-SW480 cells and (G) CR-SW620 cells. (“Con” represented “Control group”, “Cis” indicated “Cisplatin treated group”, “Cis+KD-S” represented “Cisplatin plus downregulated S100A4 treated group”, “Cis+OE-p53” represented “Cisplatin plus overexpressed p53 treated group”). All the experiments repeated at least 3 times. “*” means p < 0.05, “**” means p < 0.01 and “NS” means no statistical significance.