Research Paper Volume 11, Issue 24 pp 12328—12344

Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis


Figure 6. METTL1 regulated cell proliferation by targeting S100A4. (A, B) Colony formation assay was performed to detect cell proliferation. Western Blot was used to determine the expression levels of Cyclin D1, CDK2 and p27 in (C, D) CR-HCT116 cells, (E, F) CR-SW480 cells and (GH) CR-SW620 cells. (“Con” represented “Control group”, “Cis” indicated “Cisplatin treated group”, “Cis+OE-MET” represented “Cisplatin plus overexpressed METTL1 treated group”, “Cis+OE-M+OE-S” represented “Cisplatin plus overexpressed METTL1 and overexpressed S100A4 treated group”). All the experiments repeated at least 3 times. “*” means p < 0.05, “**” means p < 0.01 and “NS” means no statistical significance.