Research Paper Volume 11, Issue 24 pp 12361—12374

A phenolic amide (LyA) isolated from the fruits of Lycium barbarum protects against cerebral ischemia–reperfusion injury via PKCε/Nrf2/HO-1 pathway

Figure 5. Effects of LyA on apoptotic in SH-SY5Y cells. (A) Hoechst 33342 staining (scale bar 100 μm). (B) Effects of LyA on OGD-induced apoptosis after AV-PI double stain. (C) The quantitative analysis of apoptotic cells. (D) Protein expressions of bax, bcl-2 and cleaved caspase-3 were evaluated by western blot analysis. (E) Relative expressions are calculated and are shown here. Data were represented as means ± SD (n=6). *p < 0.05 compared with control group; #p < 0.05 compared with OGD group.