Research Paper Volume 11, Issue 24 pp 12361—12374

A phenolic amide (LyA) isolated from the fruits of Lycium barbarum protects against cerebral ischemia–reperfusion injury via PKCε/Nrf2/HO-1 pathway

Figure 8. PKCε mediated LyA-induced neuroprotection, Nrf2 nuclear translocation, and HO-1 upregulation. (AC) Cells were transfected with control or PKCε siRNA for 48 h, followed by treatment with 40 μM LyA for 8 h. p-PKCε, PKCε, HO-1, Nrf2, bax, bcl-2 and cleaved caspase-3 expression levels were analyzed by western blotting. Data were presented as mean ± SD (n =6). *p < 0.05 vs si-control group without LyA; #p < 0.05 vs si-control group with LyA. (D) Cells were treated for 48 h with control or siRNA, and then treated with 40 μM LyA for 8 h before being subjected to 60 min OGD followed at 24 h by the MTT assay. (E) Intracellular ROS level. Data were represented as means ± SD (n=6). *p < 0.05 compared with OGD group; #p < 0.05 compared with OGD + LyA group.