Research Paper Volume 11, Issue 24 pp 12532—12545

Protease-activated receptor 2 (PAR-2) antagonist AZ3451 as a novel therapeutic agent for osteoarthritis

Figure 4. AZ3451 attenuates IL-1β induced apoptosis in chondrocytes. (A, B) Flow cytometry of Annexin V-FITC/PI staining and quantification data of the apoptosis rates in chondrocytes as treated above. (C, D) Representative western blots and quantification data of Bcl-2, BAX, Cyto C and Cleaved caspase 3 in chondrocytes as treated above. (E, F) Representative fluorescence photographs of mitochondrial membrane potential and JC-1 monomer rates in chondrocytes as treated above. (G, H) TUNEL staining assay was performed in chondrocytes as treated above. Data are shown as the mean ± SD. Significant differences between groups are indicated as ***P <0.001, **P < 0.01 and *P < 0.05.