Research Paper Volume 11, Issue 24 pp 12532—12545

Protease-activated receptor 2 (PAR-2) antagonist AZ3451 as a novel therapeutic agent for osteoarthritis

Figure 5. CQ attenuates the anti-apoptotic effect of AZ3451 in chondrocytes. (A, B) Representative western blots and quantification data of Cleaved caspase 3 and LC3 in chondrocytes as treated above. (C, D) TUNEL staining assay was performed in chondrocytes as treated above. (E, F) Representative western blots and quantification data of collagen II, aggrecan, COX2, ADAMTS5 and MMP13 as treated above. Data are shown as the mean ± SD. Significant differences between groups are indicated as ***P <0.001, **P < 0.01 and *P < 0.05.