Research Paper Volume 11, Issue 24 pp 12532—12545

Protease-activated receptor 2 (PAR-2) antagonist AZ3451 as a novel therapeutic agent for osteoarthritis

Figure 7. AZ3451 rescues cartilage destruction in rat OA model in vivo. (A) Representative H&E, Toluidine blue and Safranin O/Fast Green staining of cartilage in three groups after 8-week post-surgery. (B) OARIS scores of cartilage in three groups. (C, D) Immunohistochemical staining assay of MMP13, Cleaved-caspase3 and Beclin1 in the rat cartilage. (E, F) TUNEL staining assay in the rat cartilage. Data are shown as the mean ± SD. Significant differences between groups are indicated as ***P <0.001, **P < 0.01 and *P < 0.05.