Research Paper Volume 11, Issue 24 pp 12600—12623

Genome-wide global identification of NRF2 binding sites in A549 non-small cell lung cancer cells by ChIP-Seq reveals NRF2 regulation of genes involved in focal adhesion pathways

Figure 4. Functional annotation and validation of novel NRF2-regulated genes using qRT-PCR. (A) Bar chart of KEGG pathways associated with the NRF2 TFBS (P<0.05). (B) NRF2-driven focal adhesion pathway genes downregulated in NRF2-knockdown A549 cells (18S rRNA served as an internal control; value for siGFP-C5 cells was set at 100%; *p<0.05, **p<0.01).