Research Paper Volume 11, Issue 24 pp 12733—12753

The loss of dopaminergic neurons in DEC1 deficient mice potentially involves the decrease of PI3K/Akt/GSK3β signaling


Figure 1. DEC1 deficient mice exhibit motor abnormalities. (AC) Locomotion activity and motor coordination were analyzed by rotarod test (RT), beam walking test (BWT) and open-field test (OFT) in DEC1+/+ and DEC1-/- mice (n=15 in each group) at the age of 3, 6 and 12 months. (A) The latency to fall off the rotated rod in RT (Two-way AONVA, gene: F(1,54)=17.767, p<0.001; age: F(2,54)=6.041, p=0.004; interaction: F(2,54)=0.297, p= 0.744). (B) Time to transverse the beam within 5 min in BWT (Two-way AONVA, gene: F(1,40)=7.354, p=0.001; age: F(2,40)=20.977, p<0.001; interaction: F(2,40)=0.288, p= 0.751). (C) Total traveled distance (Two-way AONVA, gene: F(1,49)=9.333, p=0.004; age: F(2,49)=1.266, p=0.291; interaction: F(2,49)=2.274, p= 0.114). The data are analyzed using t-test for the same age in two genotypes of mice and expressed as mean ± SD. *p<0.05, **p<0.01 vs the age-matched littermate DEC1+/+ mice. ## p<0.01, ns p>0.05, comparisons are shown in the figure.