Research Paper Volume 11, Issue 24 pp 12733—12753

The loss of dopaminergic neurons in DEC1 deficient mice potentially involves the decrease of PI3K/Akt/GSK3β signaling

Figure 6. DEC1 deficiency decreases PI3K/Akt/GSK3β pathway in the midbrain. (AC) Levels of PI3Kp110α and its downstream targets in the midbrain of two types (DEC1+/+ and DEC1-/-) of mice at the age of 3, 6 and 12 months (n=6 in each group) by Western blotting. (D) Related level of PI3Kp110α (Two-way AONVA, gene: F(1,17)=16.846, p=0.01; age: F(2,17)=1.28, p=0.303; interaction: F(2,17)=1.28, p=0.303). (E) Related level of p-Akt (Two-way AONVA, gene: F(1,18)=35.478, p<0.01; age: F(2,18)=12.597, p<0.001; interaction: F(2,18)=8.1, p=0.003). (F) Related level of p-GSK3β (Two-way AONVA, gene: F(1,17)=23.078, p<0.01; age: F(2,17)=0.635, p=0.542; interaction: F(2,17)=0.635, p=0.542). (G) Related level of β-catenin (Two-way AONVA, gene: F(1,17)=47.478, p<0.01; age: F(2,17)=6.065, p=0.01; interaction: F(2,17)=6.065, p=0.01). The data are analyzed using t-test for the same age in two genotypes of mice and expressed as mean ± SD. *p<0.05, **p<0.01 vs the age-matched DEC1+/+ mice.