Figure 4. Validation of DEGs identified in the microarray analysis. (A and B) qRT-PCR analysis of 18 DEGs in Doc-resistant DU145R and PC3R smples. (C–E) ROC curves for S100A4, ACKR3 and CDH1 in the microarray. (F–H) Correlation between the expression levels among S100A4, ACKR3 and CDH1. Expression data are represented by a log ratio calculated by comparing ΔCq from the DOC-R samples with ΔCq from the controls. ΔCq was calculated as the difference between Cq of the targeted genes and Cq of the endogenous control gene ACTB.