Figure 7. Immunofluorescent localization of KIFC1 and tubulin during post-spermiogenesis in the vas deferens and in mature sperm from spermatophore of P. japonicus. (A–E) Spermatid during post-spermiogenesis in the vas deferens. The signal of KIFC1 was very weak. The microtubules exhibited spindle morphology around the sperm nucleus (white arrows). (F–J) Mature sperm in spermatophore. KIFC1 and microtubules have completely separated localization in the mature sperm. From (J), it was clearly that KIFC1 was mainly localized at acrosome, while the microtubules distributed in the opposite side cytoplasm. Blue: DAPI, Green: tubulin, Red: KIFC1. Scale bar = 20μm.