Research Paper Volume 11, Issue 24 pp 12793—12809

Loss of Klotho contributes to cartilage damage by derepression of canonical Wnt/β-catenin signaling in osteoarthritis mice


Figure 5. CTS influenced Wnt/β-catenin signaling pathway, Klotho binds to Wnt and blocks Wnt-mediated gene transcription. Chondrocytes were incubated with or without CTS(0.1, 0.5.1 HZ) for 48 hours. the binding of Dvl to Frizzled was induced by CTS in a tension-dependent manner, Klotho to Wnt1, Wnt4, Wnt7a was adversed. (A, B) Detection of Dvl and Frizzled binding and (B) Klotho and Wnt1, 4, 7a binding in supernatant by Co-IP, quantitative data (C) is presented. *P< 0.05 versus controls (n=3). (D, E) The effect of β-catenin on the activity of MMP-2 promoters and MMP-9 promoters was in a tension dependent manner. Results were obtained via the expression of three individual experiments performed in triplicate for each condition. *P < 0.05, **P < 0.01.