Research Paper Volume 12, Issue 2 pp 1114—1127

Romo1 is involved in the immune response of glioblastoma by regulating the function of macrophages

Figure 2. The overexpression of Romo1 in bone marrow cells dampened the immune response in glioblastoma. (A) The overexpression of Romo1 in the bone marrow cells transfected with Romo1-lentivirus (the right lane) was validated by western blotting. The protein sample of the bone marrow cells transfected with control lentivirus (the left lane) was used as control. (B, C) The tumor growth curve (B) and the survival curve (C) of the mice receiving the control bone marrow allografts alone (n=6) or in combination of clodronate liposomes (200ul of each mouse every 3 days, n=6),, and the mice receiving the Romo1-overexpressed bone marrow allografts alone (n=6) in combination of in combination of clodronate liposomes (200ul of each mouse every 3 days, n=6) after orthotopic injection of GL261 cells. (D) The tumor sections were analyzed by immunohistochemistry with CD3 antibody, and the infiltrating CD3+ T cells around tumors were quantified and statistically analyzed. Arrows indicate CD3+ T cells. (E) The frequencies of IFN-γ-producing CD4+ or CD8+ T cells in tumors of both groups were determined by flow cytometry and statistically analyzed. (F) The frequencies of GzmB-producing CD4+ or CD8+ T cells in tumors of both groups were determined by flow cytometry and statistically analyzed. (G) The tumor sections from both groups were analyzed by immunohistochemistry with Ki-67 antibody and statistically analyzed from three independent experiments. Arrows indicate the Ki-67+ cells. *, P < 0.05; **, P < 0.01.