Research Paper Volume 12, Issue 2 pp 1114—1127

Romo1 is involved in the immune response of glioblastoma by regulating the function of macrophages

Figure 5. The overexpression of Romo1 promoted macrophage polarization toward M2 phenotype. (A) The control and Romo1-overexpressed macrophages were analyzed by immunofluorescence with CD206 and iNOS antibodies. The CD206+ and iNOS+ cells were quantified and statistically analyzed. (B, C) The control and Romo1-overexpressed macrophages were analyzed by flow cytometry with M1 markers (CD11c and F4/80) and M2 markers (CD206 and CD11b). The double positive cells were respectively quantified and statistically analyzed. (D) The mRNA levels of M1-related genes (IL-6, iNOS, TNF-α) and M2-related genes (Arginase 1, Ym1, IL-10) were determined by RT-qPCR in control and Romo1-overexpressed macrophages. (E) The production of IL-1β, IL-12, TGF-β or IL-23 was respectively analyzed by ELISA in control and Romo1-overexpressed macrophages. Data are representative of at least three independent experiments and are presented as mean ± SD. ns, not significant; *, P < 0.05; **, P < 0.01.