Research Paper Volume 12, Issue 2 pp 1114—1127

Romo1 is involved in the immune response of glioblastoma by regulating the function of macrophages

Figure 7. The inhibition of Romo1 enhanced the efficacy of anti-PD-1 immunotherapy in glioblastoma. (A) The Romo1 shRNA efficiency in bone marrow cells was determined by western blotting. (B, C) The tumor growth curve (B) and the survival curve (C) of the mice receiving control or Romo1 knockdown bone marrow allografts (with or without treatment of PD-1 antibody on Day 7, 10 and 13) and orthotopic injection of GL261 cells. (D) The DHE levels were analyzed by flow cytometry in control and Romo1-overexpressed (with or without treatment of PD-1 antibody) macrophages. (E, F) The control and Romo1-overexpressed (with or without treatment of PD-1 antibody on Day 7, 10 and 13) macrophages were respectively analyzed by flow cytometry with M1 (E) or M2 (F) markers. Data are representative of at least three independent experiments and are presented as mean ± SD. ns, not significant; *, P < 0.05; **, P < 0.01.