Research Paper Volume 12, Issue 2 pp 1141—1158

MiR-29b-3p promotes particulate matter-induced inflammatory responses by regulating the C1QTNF6/AMPK pathway

Figure 1. MiR-29b-3p was upregulated upon PM exposure. (A) The expression of miRNAs was measured using a inflammation-related miRNAs PCR array in HBECs with or without 300 μg/cm3 PM for 24 h. The points outside the dashed lines indicated miRNAs with fold change > 2 among the subsets (n= 2 biological replicates). (B) Real-time PCR analysis of miR-29b-3p expression in HBECs, MLE-12, and RAW264.7 cells with or without 300 μg/cm3 PM for 24 h. (C) HBECs were stimulated with different doses of PM (50, 100, 200, and 300 μg/cm3) for 24 h and the expression of miR-29b-3p was detected using real-time PCR. (D) HBECs were treated with 300 μg/cm3 PM for different durations (3, 6, 12, and 24 h) and the expression of miR-29b-3p was detected using real-time PCR. Values represent mean ± SEM; *, P<0.05, compared with the control group; n=3. HBECs, human bronchial epithelial cells; PM, particulate matter.