Figure 5. FGF10 reduced the nuclear HMGB1 release and expression of TLR4. (A) FGF10 (10 ng/ml) was used to pretreat HBECs for one hour, after which these were exposed for 24 hours in 200 μg/cm3 of PM or PBS. The HMGB1 expression was assessed via confocal microscopy. DAPI (blue) was used for the nuclear staining. Scale bars=10 μm. (B) The nuclear HMGB1 protein levels were assessed via western blot, and GAPDH was used as the loading control. (C) The supernatant HMGB1 levels were measured via ELISA. (D and E) The TLR4 expression in HBECs was assessed via western blotting and RT-qPCR. Data were presented as the mean ± standard error of the mean (SEM) of three independent experiments. *P<0.05, **P<0.01 vs. control. #P<0.05, ##P<0.01 vs. PM.