Research Paper Volume 12, Issue 2 pp 1186—1200

Fibroblast growth factor 10 alleviates particulate matter-induced lung injury by inhibiting the HMGB1-TLR4 pathway

Figure 6. The increase in HMGB1 levels compromised the cytoprotective effect of FGF10. (A and B) The recombinant HMGB1 plasmid transfection upregulated the HMGB1 expression and TLR4 activation. (CE) HMGB1-overexpressing cells were treated with FGF10, after which apoptotic death and viability were measured following PM exposure. (FH) The IL-6, IL-8 and TNF-α levels were measured via RT-qPCR. (I) The supernatant levels of IL-6, IL-8 and TNF-α obtained from the treated cells were quantified via ELISA. Data were presented as the mean ± standard error of the mean (SEM) of three independent experiments. *P<0.05, **P<0.01 vs. control. #P<0.05, ##P<0.01 vs. PM. +P<0.05, ++P<0.01 vs. PM+FGF10.