Research Paper Volume 12, Issue 2 pp 1213—1236

Figure 1. Doxorubicin-induced the simultaneous upregulation of PD-L1 and miR-200a in osteosarcoma cell lines. (A) Heat map illustrating hierarchical clustering of 36 differential miRNAs in UOSR2 and paired U2OS. (B) Volcano plot of differential miRNAs. Up-regulated miRNAs (red) and down-regulated miRNAs (green) were determined under the conditions of fold change (U2OSR2 versus U2OS) and false discovery rate-adjusted p-value ≤ 0.05. P-value was transformed by −log10. The fold change was also log-transformed. (C) Expression of microRNAs in U2OS after doxorubicin stimulation. (D–E) Western blot analysis of PD-L1 in U2OS with stimulation of different concentration of doxorubicin. (F–G) Western blot analysis of PD-L1 in K7 with stimulation of different concentration of doxorubicin. (H) qRT-PCR analysis of PD-L1 in U2OS with stimulation of different concentration of doxorubicin. (I) qRT-PCR analysis of PD-L1 in K7 with stimulation of different concentration of doxorubicin. (J) qRT-PCR analysis of miR-200a in U2OS with stimulation of different concentration of doxorubicin. (K) qRT-PCR analysis of miR-200a in K7 with stimulation of different concentration of doxorubicin. *P<0.05, **P<0.01, ***P<0.001.