Figure 2. Increased ZEB1-AS1 facilitated the viability and stemness of CCA cells. (A) The expression of ZEB1-AS1 in CCA QBC939, CCLP-1, RBE, TFK-1 cells and normal HIBEC. (B) The knockdown efficiencies of si-ZEB1-AS1-1 and si-ZEB1-AS1-2 as well as amplification efficiency of pcDNA3.1-ZEB1-AS1 were monitored through qRT-PCR. (C) CCK-8 proliferation curves were drawn to show the effect of ZEB1-AS1 on cellular proliferation. (D) The red stains representing proliferative activity were reduced in QBC939 and CCLP-1 cells transfected with si-ZEB1-AS1-1 and si-ZEB1-AS1-2. (E) The cell colonies were decreased in si-ZEB1-AS1 cells revealed by colony formation assays. (F) Spheroid forming abilities of QBC939 and CCLP-1 cells transfected with si-ZEB1-AS1-1 and si-ZEB1-AS1-2 were restrained. *P < 0.05, **P < 0.01, ***P < 0.001.