AR-induced ZEB1-AS1 represents poor prognosis in cholangiocarcinoma and facilitates tumor stemness, proliferation and invasion through mediating miR-133b/HOXB8

Xingming Jiang; Jinglin Li; Weina Wang; Zengtao Hu; Canghai Guan; Yuqiao Zhao; Wenzhi Li; Yunfu Cui

doi:doi:10.18632/aging.102680

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Research Paper Volume 12, Issue 2 pp 1237—1255

AR-induced ZEB1-AS1 represents poor prognosis in cholangiocarcinoma and facilitates tumor stemness, proliferation and invasion through mediating miR-133b/HOXB8

Figure 3. Upregulated ZEB1-AS1 promoted cellular migration and invasion through promoted EMT process. (A) The wound closure of QBC939 and CCLP-1 cells transfected with si-ZEB1-AS1-1 and si-ZEB1-AS1-2 was delayed verified by wound healing assays. (B) The numbers of migrating cells were decreased in si-ZEB1-AS1 cells. (C) Transwell assays displayed that knocking down ZEB1-AS1 inhibited invasion of QBC939 and CCLP-1 cells compared with controls. (D) EMT-related proteins including epithelial marker (E-cadherin) and mesenchymal markers (snail and vimentin) were measured via western blot. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.