Figure 3. Upregulated ZEB1-AS1 promoted cellular migration and invasion through promoted EMT process. (A) The wound closure of QBC939 and CCLP-1 cells transfected with si-ZEB1-AS1-1 and si-ZEB1-AS1-2 was delayed verified by wound healing assays. (B) The numbers of migrating cells were decreased in si-ZEB1-AS1 cells. (C) Transwell assays displayed that knocking down ZEB1-AS1 inhibited invasion of QBC939 and CCLP-1 cells compared with controls. (D) EMT-related proteins including epithelial marker (E-cadherin) and mesenchymal markers (snail and vimentin) were measured via western blot. *P < 0.05, **P < 0.01, ***P < 0.001.