Figure 7. ZEB1-AS1 promoted malignant progression of CCA through mediating miR-133b/HOXB8. (A, B) HOXB8 downexpression caused by ZEB1-AS1 knockdown was saved by silencing miR-133b. (C–E) Rescue assays of EdU, spheroid formation and transwell confirmed that inhibition of proliferation, stemness and invasion induced by knocking down ZEB1-AS1 was saved through silencing miR-133b, respectively. (F–H) CCLP-1 cells cotransfected with pcDNA3.1-ZEB1-AS1 and sh-HOXB8 were used to carry out EdU, spheroid formation and transwell assays, respectively. (I–K) Restoration of HOXB8 expression rescued the promotion of proliferation, stemness and invasion generated through miR-133b knockdown in EdU, spheroid formation and transwell assays, respectively. *P < 0.05, **P < 0.01, ***P < 0.001.