Figure 8. ZEB1-AS1/miR-133b/HOXB8 contributed to CCA tumorigenesis in vivo. (A) Xenograft tumors were resected on the 21st day after injection. (B) Tumor volumes were calculated every 3 days throughout the course of tumor growth. (C) CCLP-1 cells cotransfected with sh-ZEB1-AS1 and antagomir-133b were subcutaneously injected into the posterior flanks of mice. (D) Tumor weights were measured after excision. (E, F) HOXB8 expression in xenograft tumors of the three groups (sh-NC/antagomir-NC, sh-ZEB1-AS1/antagomir-NC, sh-ZEB1-AS1/antagomir-133b). *P < 0.05, **P < 0.01, ***P < 0.001.