Research Paper Volume 12, Issue 2 pp 1256—1271

Ubiquinol-10 delays postovulatory oocyte aging by improving mitochondrial renewal in pigs

Figure 5. Ubiquinol-10 rescued aging-induced compromise of mitophagy. Immunofluorescence images (A) and relative fluorescence intensity (B) showing PINK1 expression in 0 h, Ctrl-24 h, Ubi-24 h, Ctrl-48 h, and Ubi-48 h oocytes. Scale bars indicate 20 μm. Protein levels of PINK1 (C and D) in 0 h, Ctrl-24 h, and Ubi-24 h oocytes were confirmed by western blotting. Immunofluorescence images (E) and relative fluorescence intensity (F) showing PARKIN expression in 0 h, Ctrl-24 h, Ubi-24 h, Ctrl-48 h, and Ubi-48 h oocytes. Scale bars indicate 20 μm.*p < 0.05 and ***p < 0.001 indicate significant differences.